Optimization of conditions to extract high quality DNA for PCR analysis from whole blood using SDS-proteinase K method
نویسندگان
چکیده
منابع مشابه
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Department of Medical Microbiology, University of Z/irich, 8028 Z/irich, Switzerland A large number of different protocols for the efficient isolation of highly purified DNA from eukaryotic and prokaryotic cells is extant. (1-4) These procedures usually include treatment with proteinase K in the presence of SDS, which efficiently lyses the cells and nuclei and liberates the DNA tightly bound in...
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Typically DNA used in PCR assays is usually extracted according to die phenol-chloroform method (1) or by an alternative 'saltingout' rapid purification (2). Moreover partially purified DNA obtained with rapid procedures have been reported to be suitable for PCR amplification (3). We describe here a more simple and efficient method to amplify DNA directly from whole blood samples without any pu...
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ژورنال
عنوان ژورنال: Saudi Journal of Biological Sciences
سال: 2017
ISSN: 1319-562X
DOI: 10.1016/j.sjbs.2016.09.016